Fig 1: IL4 on C2C12 reduces the TNFα‐induced proliferative effect and is essential for myotube differentiation. (A) IF, (B) flow cytometry analysis, and (C) quantification of Ki67+ C2C12 cells treated with 10 ng/mL IL4, 100 ng/mL TNFα, or a combination of the two cytokines (TNFα + IL4) during 48 h of proliferating medium. (D) WB and (E) quantifications of the protein levels of MyHC and IL4R1a normalized for aTub after 10 ng/mL IL4 or 100 ng/mL TNFα or a combination of the two (TNFα + IL4) treatments during all C2C12 differentiation period (5 days differentiating medium). (F) IF for MyHC (red) on C2C12 during the three last days of differentiation (24 h = Day 3 of differentiation, 48 h = Day 4 of differentiation, 72 h = Day 5 of differentiation) after the double IL4Ra and IL13Ra silencing. HOECHST (blue) was used to stain nuclei. (G) WB and (H, I) quantifications for MyHC and IL4Ra protein levels normalized for aTub after double IL4Ra and IL13Ra silencing. (J) IF, (K) WB, and (L) quantification for the levels of ESGP (Myomerger) normalized on the ones of Gapdh that were analysed in the same cells silenced for IL4Ra and IL13Ra. Significance of the differences: * P < 0.05, ** P < 0.01, *** P < 0.001 vs. C; + P < 0.05, ++ P < 0.01, +++ P < 0.001 vs. IL4.
Supplier Page from MilliporeSigma for Anti-IL-4R antibody produced in rabbit